Search results for "ADH II"

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Zymomonas mobilis dzelzi saturošā alkoholdehidrogenāzes izoenzīma loma oksidatīvajā stresā

2021

Darbā tika analizēta ūdeņraža peroksīda rezistence Z. mobilis mutantu celmos ar izmainītu elpošanas ķēdes darbību, izslēgtu ADH II un/vai katalāzi: Zm6-ndh, Zm6-ndh/pNDH, Zm6-adhB, Zm6-adhB-ndh, Zm6-cat, Zm6-adhB-cat, lai gūtu ieskatu par ADH II lomu ūdeņraža peroksīda izraisītajā stresā. Salīdzinot mutantu celmu augšanu pie dažādām H2O2 koncentrācijām konstatēts, ka visaugstāko izturību pret peroksīdu izrāda Zm6-adhB, Zm6-adhB-ndh celmi, kuriem bija ievērojami paaugstināta katalāzes aktivitāte. Katalāzes negatīvajiem celmiem Zm6-cat, Zm6-adhB-cat, kuriem katalāzes nav, bija ļoti zema peroksīda rezistence, bet starp abiem celmiem tā neatšķīrās. Tas liecināja ka katalāzes pieaugums ir izšķir…

elpošanas ķēdeZymomonas mobilisADH IIūdeņraža peroksīdsBioloģija

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